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Neutralising Pns11-sperm binding To detect the direct interaction between Pns11 and Pelo on sperms, the mature live sperms excised from nonviruliferous males were pre-incubated with pre-immune or Pelo antibody (0.5 g/L) for 30 min and then incubated with purified Pns11 proteins (1.0 g/L) for 1 h
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